Journal: Genetics and molecular research : GMR

Article Title: Increased survivin expression and its association with clinical parameters of congenital choledochal cysts.

doi: 10.4238/gmr.15028032

Figure Lengend Snippet: Figure 1. Immunohistochemical staining results for the protein expression of survivin. The cytoplasm showed high survivin expression, which presented as granular form, with yellow or dark brown staining, while the nuclei were hardly stained (A. cytoplasm, 200X; B. nuclei, 400X).

Article Snippet: The primary mouse anti-human survivin monoclonal antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (China).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Molecular Cancer

Article Title: Flavokawain B, a kava chalcone, inhibits growth of human osteosarcoma cells through G2/M cell cycle arrest and apoptosis

doi: 10.1186/1476-4598-12-55

Figure Lengend Snippet: The apoptotic effect of FKB on OS cells. A , 143B cells were treated with different concentrations of FKB for 24 h. Apoptosis was evaluated by DAPI staining. B , 143B and Saos-2 cells were stained with annexin V and propidium iodide and analyzed by flow-cytometry. C , The chart illustrates the results from three separate experiments of flow-cytomety. D , FKB treatment induced the expression of Fas, Bax, Puma, and decreased Survivin and Bcl-2 expression. Cells were treated for 24 h and protein was resolved by SDS-PAGE with GAPDH as a control.

Article Snippet: Primary antibodies for Survivin, Bax, Bcl-2, Bcl-xl, Fas, cdc25c, Myt1, cdc2, P-cdc2, Wee1, CyclinB1, P-Chk1(345), GAPDH and secondary antibodies were purchased from Cell Signaling Technology, and antibodies against MMP-2 and MMP-9 were obtained from Thermo Scientific.

Techniques: Staining, Flow Cytometry, Expressing, SDS Page, Control

Journal: Cells

Article Title: An Insight into Survivin in Relevance to Hematological, Biochemical and Genetic Characteristics in Tobacco Chewers with Oral Squamous Cell Carcinoma

doi: 10.3390/cells12101444

Figure Lengend Snippet: Photomicrographs of immunohistochemical staining for survivin in oral squamous cell carcinoma tissues ( A – D ) and normal buccal mucosa ( E , F ). Immunohistochemical staining shows positive staining in brown. Photographs captured with a digital camera at 100× and 400× magnification under identical conditions.

Article Snippet: Antigen retrieval was performed with citrate buffer pH 9 using a decloaker, primary mouse monoclonal antibodies against survivin (IHC 668, GenomeMe, GenomeMe Lab Inc., Richmond, BC, Canada), and a secondary antibody linked to horseradish peroxidase, which specifically binds to the primary antibody.

Techniques: Immunohistochemical staining, Staining

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo.

doi: 10.1016/j.ymthe.2004.05.006

Figure Lengend Snippet: FIG. 1. The effects of plasmid vectors encoding U6 promoter-driven siRNA against survivin on survivin expression in 293T cells. 293T cells (8 105) were seeded onto 60-mm dishes. Sixteen hours later, the seeded cells were transfected with 6 Ag DNA by lipofection. (A) RT-PCR analysis of survivin or GAPDH (internal control) mRNA expression performed 72 h after transfection. (B) Immunoblot analysis of survivin, XIAP, or h-actin (internal control) protein expression performed 72 h after transfection. pUC19 and psiGL3B are control vectors encoding either no expression unit or siRNA against firefly luciferase. psiSurv(A) and psiSurv(B) are the vectors that express siRNA against different sites of the survivin transcript. The survivin levels remaining were calculated as % control relative to loading control.

Article Snippet: The primary antibody was mouse anti-survivin monoclonal antibody (2802; Cell Signaling Technology, Beverly, MA, USA), rabbit anti-XIAP polyclonal antibody (X-2503; Sigma, St. Louis, MO, USA), rabbit anti-caspase-3 polyclonal antibody (9662; Cell Signaling Technology), or mouse anti-h-actin monoclonal antibody (A-5441; Sigma).

Techniques: Plasmid Preparation, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, Luciferase

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo.

doi: 10.1016/j.ymthe.2004.05.006

Figure Lengend Snippet: FIG. 6. The impact of adenoviral vectors encoding siRNA against survivin on tumor- igenicity of HeLa cells in vivo. Adenoviral infection was performed at 3000 pt/cell. Twenty-four hours later, 5 106 infected cells were injected subcutaneously into a nude mouse. The tumor growth curves from individual animals are shown. Mice were humanely killed following development of a tumor larger than 2000 mm3 or a tumor harboring an ulcer.

Article Snippet: The primary antibody was mouse anti-survivin monoclonal antibody (2802; Cell Signaling Technology, Beverly, MA, USA), rabbit anti-XIAP polyclonal antibody (X-2503; Sigma, St. Louis, MO, USA), rabbit anti-caspase-3 polyclonal antibody (9662; Cell Signaling Technology), or mouse anti-h-actin monoclonal antibody (A-5441; Sigma).

Techniques: In Vivo, Infection, Injection

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo.

doi: 10.1016/j.ymthe.2004.05.006

Figure Lengend Snippet: FIG. 5. The impact of adenoviral vectors encoding siRNA against survivin on growth of HeLa, U251, and MCF-7 cells in vitro. Adenoviral infection was performed at 3000 pt/cell. Twenty-four hours after infection, cells were seeded onto six-well culture plates at a concentration of 102 or 103 cells per well and were cultured for 12 days. (A) Photographs of the colonies stained by methylene blue. The experiments were performed in triplicate and representative data are shown. (B) The means F SD of the A655, as determined by colori- metric analysis, for the colonies from three determinations are shown. The experiments were repeated twice and similar results were obtained. Representative data are shown.

Article Snippet: The primary antibody was mouse anti-survivin monoclonal antibody (2802; Cell Signaling Technology, Beverly, MA, USA), rabbit anti-XIAP polyclonal antibody (X-2503; Sigma, St. Louis, MO, USA), rabbit anti-caspase-3 polyclonal antibody (9662; Cell Signaling Technology), or mouse anti-h-actin monoclonal antibody (A-5441; Sigma).

Techniques: In Vitro, Infection, Concentration Assay, Cell Culture, Staining

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo.

doi: 10.1016/j.ymthe.2004.05.006

Figure Lengend Snippet: FIG. 7. The impact of adenoviral vectors encoding siRNA against survivin on growth of preestablished tumor in U251 xenograft model. Intratumoral injection of adenoviral vectors was begun when the tumor size reached about 5 to 7 mm in diameter. Each treatment (1010 pt/tumor) is shown as an arrow. The mean F SD of the tumor volume for each group (n = 6) is shown for each time point. Mice were humanely killed following development of a tumor larger than 2000 mm3.

Article Snippet: The primary antibody was mouse anti-survivin monoclonal antibody (2802; Cell Signaling Technology, Beverly, MA, USA), rabbit anti-XIAP polyclonal antibody (X-2503; Sigma, St. Louis, MO, USA), rabbit anti-caspase-3 polyclonal antibody (9662; Cell Signaling Technology), or mouse anti-h-actin monoclonal antibody (A-5441; Sigma).

Techniques: Injection